Expression of poplar phenylalanine ammonia-lyase in insect cell cultures.
Identifieur interne : 004A49 ( Main/Exploration ); précédent : 004A48; suivant : 004A50Expression of poplar phenylalanine ammonia-lyase in insect cell cultures.
Auteurs : G R Mckegney [Canada] ; S L Butland ; D. Theilmann ; B E EllisSource :
- Phytochemistry [ 0031-9422 ] ; 1996.
Descripteurs français
- KwdFr :
- ADN complémentaire (MeSH), Animaux (MeSH), Arbres (enzymologie), Baculoviridae (MeSH), Données de séquences moléculaires (MeSH), Délétion de séquence (MeSH), Expression des gènes (MeSH), Lignée cellulaire (MeSH), Mutagenèse dirigée (MeSH), Oligodésoxyribonucléotides (MeSH), Phenylalanine ammonia-lyase (biosynthèse), Phenylalanine ammonia-lyase (isolement et purification), Protéines recombinantes (biosynthèse), Protéines recombinantes (isolement et purification), Sites de fixation (MeSH), Spodoptera (MeSH), Séquence nucléotidique (MeSH), Sérine (MeSH), Transfection (MeSH), Vecteurs génétiques (MeSH), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
- biosynthèse : Phenylalanine ammonia-lyase, Protéines recombinantes.
- enzymologie : Arbres.
- isolement et purification : Phenylalanine ammonia-lyase, Protéines recombinantes.
- ADN complémentaire, Animaux, Baculoviridae, Données de séquences moléculaires, Délétion de séquence, Expression des gènes, Lignée cellulaire, Mutagenèse dirigée, Oligodésoxyribonucléotides, Sites de fixation, Spodoptera, Séquence nucléotidique, Sérine, Transfection, Vecteurs génétiques, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Animals (MeSH), Baculoviridae (MeSH), Base Sequence (MeSH), Binding Sites (MeSH), Cell Line (MeSH), DNA, Complementary (MeSH), Electrophoresis, Polyacrylamide Gel (MeSH), Gene Expression (MeSH), Genetic Vectors (MeSH), Molecular Sequence Data (MeSH), Mutagenesis, Site-Directed (MeSH), Oligodeoxyribonucleotides (MeSH), Phenylalanine Ammonia-Lyase (biosynthesis), Phenylalanine Ammonia-Lyase (isolation & purification), Recombinant Proteins (biosynthesis), Recombinant Proteins (isolation & purification), Sequence Deletion (MeSH), Serine (MeSH), Spodoptera (MeSH), Transfection (MeSH), Trees (enzymology).
- MESH :
- chemical , biosynthesis : Phenylalanine Ammonia-Lyase, Recombinant Proteins.
- chemical , isolation & purification : Phenylalanine Ammonia-Lyase, Recombinant Proteins.
- chemical : DNA, Complementary, Oligodeoxyribonucleotides, Serine.
- enzymology : Trees.
- Animals, Baculoviridae, Base Sequence, Binding Sites, Cell Line, Electrophoresis, Polyacrylamide Gel, Gene Expression, Genetic Vectors, Molecular Sequence Data, Mutagenesis, Site-Directed, Sequence Deletion, Spodoptera, Transfection.
Abstract
A cDNA encoding one of the phenylalanine ammonia-lyase genes from Populus trichocarpa x deltoides was inserted into a baculovirus expression vector and the PAL protein was successfully expressed in insect cell cultures. High levels of active holoenzyme were obtained that could be purified in a single chromatographic step. Site-directed mutagenesis and expression of the mutant enzyme confirmed that conversion of the putative active site serine202 residue to alanine is sufficient to destroy the catalytic activity of PAL.
DOI: 10.1016/0031-9422(95)00677-x
PubMed: 8729456
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Expression of poplar phenylalanine ammonia-lyase in insect cell cultures.</title><author><name sortKey="Mckegney, G R" sort="Mckegney, G R" uniqKey="Mckegney G" first="G R" last="Mckegney">G R Mckegney</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Science, University of British Columbia, Vancouver, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Plant Science, University of British Columbia, Vancouver</wicri:regionArea><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique</region></placeName><orgName type="university">Université de la Colombie-Britannique</orgName></affiliation></author><author><name sortKey="Butland, S L" sort="Butland, S L" uniqKey="Butland S" first="S L" last="Butland">S L Butland</name></author><author><name sortKey="Theilmann, D" sort="Theilmann, D" uniqKey="Theilmann D" first="D" last="Theilmann">D. Theilmann</name></author><author><name sortKey="Ellis, B E" sort="Ellis, B E" uniqKey="Ellis B" first="B E" last="Ellis">B E Ellis</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:8729456</idno><idno type="pmid">8729456</idno><idno type="doi">10.1016/0031-9422(95)00677-x</idno><idno type="wicri:Area/Main/Corpus">004A53</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004A53</idno><idno type="wicri:Area/Main/Curation">004A53</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004A53</idno><idno type="wicri:Area/Main/Exploration">004A53</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Expression of poplar phenylalanine ammonia-lyase in insect cell cultures.</title><author><name sortKey="Mckegney, G R" sort="Mckegney, G R" uniqKey="Mckegney G" first="G R" last="Mckegney">G R Mckegney</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Science, University of British Columbia, Vancouver, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Plant Science, University of British Columbia, Vancouver</wicri:regionArea><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique</region></placeName><orgName type="university">Université de la Colombie-Britannique</orgName></affiliation></author><author><name sortKey="Butland, S L" sort="Butland, S L" uniqKey="Butland S" first="S L" last="Butland">S L Butland</name></author><author><name sortKey="Theilmann, D" sort="Theilmann, D" uniqKey="Theilmann D" first="D" last="Theilmann">D. Theilmann</name></author><author><name sortKey="Ellis, B E" sort="Ellis, B E" uniqKey="Ellis B" first="B E" last="Ellis">B E Ellis</name></author></analytic><series><title level="j">Phytochemistry</title><idno type="ISSN">0031-9422</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Baculoviridae (MeSH)</term><term>Base Sequence (MeSH)</term><term>Binding Sites (MeSH)</term><term>Cell Line (MeSH)</term><term>DNA, Complementary (MeSH)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Gene Expression (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mutagenesis, Site-Directed (MeSH)</term><term>Oligodeoxyribonucleotides (MeSH)</term><term>Phenylalanine Ammonia-Lyase (biosynthesis)</term><term>Phenylalanine Ammonia-Lyase (isolation & purification)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (isolation & purification)</term><term>Sequence Deletion (MeSH)</term><term>Serine (MeSH)</term><term>Spodoptera (MeSH)</term><term>Transfection (MeSH)</term><term>Trees (enzymology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (MeSH)</term><term>Animaux (MeSH)</term><term>Arbres (enzymologie)</term><term>Baculoviridae (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Délétion de séquence (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Lignée cellulaire (MeSH)</term><term>Mutagenèse dirigée (MeSH)</term><term>Oligodésoxyribonucléotides (MeSH)</term><term>Phenylalanine ammonia-lyase (biosynthèse)</term><term>Phenylalanine ammonia-lyase (isolement et purification)</term><term>Protéines recombinantes (biosynthèse)</term><term>Protéines recombinantes (isolement et purification)</term><term>Sites de fixation (MeSH)</term><term>Spodoptera (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Sérine (MeSH)</term><term>Transfection (MeSH)</term><term>Vecteurs génétiques (MeSH)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Phenylalanine Ammonia-Lyase</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Phenylalanine Ammonia-Lyase</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term><term>Oligodeoxyribonucleotides</term><term>Serine</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Phenylalanine ammonia-lyase</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arbres</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Phenylalanine ammonia-lyase</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Baculoviridae</term><term>Base Sequence</term><term>Binding Sites</term><term>Cell Line</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Gene Expression</term><term>Genetic Vectors</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Sequence Deletion</term><term>Spodoptera</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Animaux</term><term>Baculoviridae</term><term>Données de séquences moléculaires</term><term>Délétion de séquence</term><term>Expression des gènes</term><term>Lignée cellulaire</term><term>Mutagenèse dirigée</term><term>Oligodésoxyribonucléotides</term><term>Sites de fixation</term><term>Spodoptera</term><term>Séquence nucléotidique</term><term>Sérine</term><term>Transfection</term><term>Vecteurs génétiques</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A cDNA encoding one of the phenylalanine ammonia-lyase genes from Populus trichocarpa x deltoides was inserted into a baculovirus expression vector and the PAL protein was successfully expressed in insect cell cultures. High levels of active holoenzyme were obtained that could be purified in a single chromatographic step. Site-directed mutagenesis and expression of the mutant enzyme confirmed that conversion of the putative active site serine202 residue to alanine is sufficient to destroy the catalytic activity of PAL.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8729456</PMID><DateCompleted><Year>1996</Year><Month>10</Month><Day>24</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0031-9422</ISSN><JournalIssue CitedMedium="Print"><Volume>41</Volume><Issue>5</Issue><PubDate><Year>1996</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Expression of poplar phenylalanine ammonia-lyase in insect cell cultures.</ArticleTitle><Pagination><MedlinePgn>1259-63</MedlinePgn></Pagination><Abstract><AbstractText>A cDNA encoding one of the phenylalanine ammonia-lyase genes from Populus trichocarpa x deltoides was inserted into a baculovirus expression vector and the PAL protein was successfully expressed in insect cell cultures. High levels of active holoenzyme were obtained that could be purified in a single chromatographic step. Site-directed mutagenesis and expression of the mutant enzyme confirmed that conversion of the putative active site serine202 residue to alanine is sufficient to destroy the catalytic activity of PAL.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>McKegney</LastName><ForeName>G R</ForeName><Initials>GR</Initials><AffiliationInfo><Affiliation>Department of Plant Science, University of British Columbia, Vancouver, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Butland</LastName><ForeName>S L</ForeName><Initials>SL</Initials></Author><Author ValidYN="Y"><LastName>Theilmann</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Ellis</LastName><ForeName>B E</ForeName><Initials>BE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>452VLY9402</RegistryNumber><NameOfSubstance UI="D012694">Serine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.3.1.24</RegistryNumber><NameOfSubstance UI="D010650">Phenylalanine Ammonia-Lyase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>B</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016367" MajorTopicYN="N">Baculoviridae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001665" MajorTopicYN="N">Binding Sites</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016297" MajorTopicYN="N">Mutagenesis, Site-Directed</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010650" MajorTopicYN="N">Phenylalanine Ammonia-Lyase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017384" MajorTopicYN="N">Sequence Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012694" MajorTopicYN="N">Serine</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018411" MajorTopicYN="N">Spodoptera</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>3</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1996</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1996</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8729456</ArticleId><ArticleId IdType="pii">003194229500677X</ArticleId><ArticleId IdType="doi">10.1016/0031-9422(95)00677-x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique</li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Butland, S L" sort="Butland, S L" uniqKey="Butland S" first="S L" last="Butland">S L Butland</name><name sortKey="Ellis, B E" sort="Ellis, B E" uniqKey="Ellis B" first="B E" last="Ellis">B E Ellis</name><name sortKey="Theilmann, D" sort="Theilmann, D" uniqKey="Theilmann D" first="D" last="Theilmann">D. Theilmann</name></noCountry><country name="Canada"><region name="Colombie-Britannique"><name sortKey="Mckegney, G R" sort="Mckegney, G R" uniqKey="Mckegney G" first="G R" last="Mckegney">G R Mckegney</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004A49 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004A49 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:8729456
|texte= Expression of poplar phenylalanine ammonia-lyase in insect cell cultures.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:8729456" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |